ERC consolidator project – BEAL
Bioenergetics in microalgae : regulation modes of mitochondrial respiration, photosynthesis, and fermentative pathways, and their interactions in secondary algae
ATP is the universal chemical energy carrier in living cells. In photosynthetic eukaryotes, it is produced by two major cellular processes: photosynthesis and respiration taking place in chloroplasts and mitochondria, respectively. Both processes support the production of biomass and govern gas (O2 and CO2) exchanges. On the other hand, anaerobic fermentative enzymes have also been identified in several primary and secondary algae. The regulation modes and interactions of respiration, photosynthesis and fermentation are fairly well understood in primary green algae. Conversely, the complex evolutionary history of secondary algae implies a great variety of original regulatory mechanisms that have been barely investigated to date.
Over the last years my laboratory has developed and optimized a range of multidisciplinary approaches that now allow us, within the frame of the BEAL (BioEnergetics in microALgae) project, to (i) characterize and compare the photosynthetic regulation modes by biophysical approaches, (ii) use genetic and biochemical approaches to gain fundamental knowledge on aerobic respiration and anaerobic fermentative pathways, and (iii) investigate and compare interconnections between respiration, photosynthesis, and fermentation in organisms resulting from distinct evolutionary scenarios. On a long term, these developments will be instrumental to unravel bioenergetics constraints on growth in microalgae, a required knowledge to exploit the microalgal diversity in a biotechnological perspective, and to understand the complexity of the marine phytoplankton.
Pierre CARDOL, PhD
FRS-FNRS Senior Research Associate
Tél. : +32 (0) 43 66 38 40
Photosynthesis and respiration in Symbiodinium and Euglena gracilis
Décrypter les processus bioénergétiques des microalgues (Uliege news, 26/02/16)
Dang KV, Pierangelini M, Roberty S, Cardol P : Alternative photosynthetic electron transfers and bleaching phenotypes upon acute heat stress in Symbiodinium and Breviolum spp.(Symbiodiniaceae) in culture. Frontiers in Marine Science 2019 6:656 DOI=10.3389/fmars.2019.00656
Vega de Luna F, Dang KV, Cardol M, Roberty S, Cardol P: Photosynthetic capacity of the endosymbiotic dinoflagellate Cladocopium sp. is preserved during digestion of its jellyfish host Mastigias papua by the anemone Entacmaea medusivora. FEMS Microbiol Ecol. 2019 Oct 1;95(10). pii: fiz141. doi: 10.1093/femsec/fiz141.
Berne N, Fabryova T, Istaz B, Cardol P, Bailleul B : The peculiar NPQ regulation in the stramenopile Phaeomonas sp. challenges the xanthophyll cycle dogma.
Biochim Biophys Acta. 2018 Jul;1859(7):491-500.
Miranda Astudillo H, Colina-Tenorio L, Jiménez-Suárez A, Vázquez-Acevedo M, Salin B, Giraud M-F, Boekema EJ, Cardol P, González-Halphen D : Oxidative phosphorylation supercomplexes and respirasome reconstitution of the colorless alga Polytomella sp.
Biochim Biophys Acta. 2018 Jun;1859(6):434-444.
Miranda-Astudillo H, Yadav S, Colina-Tenorio L, Bouillenne F, Degand H, Morsomme P, Boekema E J, Cardol P : The atypical subunit composition of respiratory complexes I and IV is associated with original extra structural domains in Euglena gracilis.
Sci Rep. 2018 Jun 26;8(1):9698.
Massoz S, Hanikenne M, Bailleul B, Coosemans N, Radoux M, Miranda Astudillo H, Cardol P, Larosa V, Remacle C : In vivo chlorophyll fluorescence screening allows the isolation of a Chlamydomonas mutant defective for NDUFAF3, an assembly factor involved in mitochondrial complex I assembly.
Plant J. 2017 Nov;92(4):584-595.
Cardol P, Krieger-Liskay A : From light capture to metabolic needs, oxygenic photosynthesis is an ever-expanding field of study in plants, algae and cyanobacteria.
Physiol Plant. 2017 Sep;161(1):2-5.
Sánchez-Vásquez L, Vázquez-Acevedo M, de la Mora J, Vega-deLuna F, Cardol P, Remacle C, Dreyfus G, González-Halphen D. Near-neighbor interactions of the membrane-embedded subunits of the mitochondrial ATP synthase of a chlorophycean alga.
Biochim Biophys Acta. 2017 Jul;1858(7):497-509.
Yadav S, Miranda-Astudillo H, Colina-Tenorio L, Bouillenne F, Degand H, Morsomme P, González-Halphen D, Boekema EJ, Cardol P : Atypical composition and structure of the mitochondrial dimeric ATP synthase from Euglena gracilis.
Biochimica et Biophysica Acta-Bioenergetics 2017 Apr;1858(4):267-275.
Emonds-Alt B, Coosemans N, Gerards T, Remacle C, Cardol P : Isolation and characterization of mutants corresponding to the MENA, MENB, MENC, and MENE enzymatic steps of 5′-monohydroxyphylloquinone biosynthesis in Chlamydomonas reinhardtii.
The Plant Journal 2017 Jan; 89(1): 141–154.